An Unbalanced Exon-Expression qPCR-based Assay for Detection of ALK Translocation (Fusion) in Lung Cancer
نویسندگان
چکیده
1National Research Council Halifax, 1411 Oxford Street, Halifax, NS B3H 3Z1, Canada 2Department of Pathology, Queen Elizabeth II Health Science Center and Dalhousie University, 5788 University Ave., Halifax, NS B3H 1V8, Canada *Corresponding author Rama K. Singh, PhD Human Health Therapeutics Portfolio National Research Council Canada 1411 Oxford Street Halifax, Nova Scotia B3H 3Z1, Canada Tel. (902) 426-3802 Fax: (902) 426-9413 E-mail: [email protected]
منابع مشابه
The use of quantitative real-time reverse transcriptase PCR for 5' and 3' portions of ALK transcripts to detect ALK rearrangements in lung cancers.
PURPOSE Approximately 3% to 7% of non-small cell lung cancers (NSCLC) harbor an ALK fusion gene, thus defining a tumor group that may be responsive to targeted therapy. The breakpoint in ALK consistently occurs at exon 20 and EML4 or other fusion partners, thus driving a strong expression of ALK kinase domain and resulting in an unbalanced expression in 5' and 3' portions of ALK transcripts. We...
متن کاملExon array profiling detects EML4-ALK fusion in breast, colorectal, and non-small cell lung cancers.
The echinoderm microtubule-associated protein-like 4-anaplastic lymphoma kinase (EML4-ALK) fusion gene has been identified as an oncogene in a subset of non-small cell lung cancers (NSCLC). We used profiling of cancer genomes on an exon array to develop a novel computational method for the global search of gene rearrangements. This approach led to the detection of EML4-ALK fusion in breast and ...
متن کاملSEC31A-ALK Fusion Gene in Lung Adenocarcinoma
Anaplastic lymphoma kinase (ALK) fusion is a common mechanism underlying pathogenesis of non-small cell lung carcinoma (NSCLC) where these rearrangements represent important diagnostic and therapeutic targets. In this study, we found a new ALK fusion gene, SEC31A-ALK, in lung carcinoma from a 53-year-old Korean man. The conjoined region in the fusion transcript was generated by the fusion of SE...
متن کاملMultiplex reverse transcription-PCR screening for EML4-ALK fusion transcripts.
PURPOSE EML4-ALK is a fusion-type protein tyrosine kinase that is generated by inv(2)(p21p23) in the genome of non-small cell lung cancer (NSCLC). To allow sensitive detection of EML4-ALK fusion transcripts, we have now developed a multiplex reverse transcription-PCR (RT-PCR) system that captures all in-frame fusions between the two genes. EXPERIMENTAL DESIGN Primers were designed to detect a...
متن کاملDetection of ALK fusion transcripts in FFPE lung cancer samples by NanoString technology
BACKGROUND ALK-rearranged lung cancers exhibit specific pathologic and clinical features and are responsive to anti-ALK therapies. Therefore, the detection of ALK-rearrangement is fundamental for personalized lung cancer therapy. Recently, new molecular techniques, such as NanoString nCounter, have been developed to detect ALK fusions with more accuracy and sensitivity. METHODS In the present...
متن کامل